Detection of mecA gene-associated methicillin-resistance coagulase-negative staphylococci (MRCoNS) from bovine mastitis in Gujarat

Exploration of pathogenic potential Coagulase-Negative Staphylococci (CoNS), in human as well animals,gained the importance during past two decades. Emergence Methicillin-Resistant Coagulase-NegativeStaphylococci (MRCoNS) bovine milk poses to be a major public health concern. Presence mecA gene isone most common reasons reported for methicillin resistant bacteria. Since scientific data concerningthe presence and MRCoNS is very limited India, particularly from Gujarat state,the present study was planned detect 25 antibiogram 33 CoNS isolatedfrom 185 mastitis cases between January 2018 December 2019. were isolated themilk samples with prevalence 38.9% (72/185), out which 39 (54.16%) (45.83%)isolates identified coagulase-positive (CoPS) coagulase-negative (CoNS),respectively. Among CoNS, S. xylosus predominantly species (9/33, 27.27%), followedby epidermis (6/33, 18.18%) haemolyticus (4/33, 12.12%). Out these 21 (63.63%)and (75.75%) by disc diffusion method CHROM agar, respectively. Contraryto likelihood, only 3 showed using PCR method. Antibiogram revealedthat isolates (84.85%) multi-drug emphasizing urgent need restricting theindiscriminate use antimicrobial drugs area study.